Journal: Cell systems
Article Title: A Predictive 3D Multi-Scale Model of Biliary Fluid Dynamics in the Liver Lobule
doi: 10.1016/j.cels.2017.02.008
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat polyclonal antibody against CD13 Acris Antibodies Cat#SM2298P Rabbit polyclonal antibody against phospho-myosin light chain (pMLC) Cell Signaling Technologies Cat#3671 Mouse polyclonal antibody against GAPDH Sigma-Aldrich Cat#G8795 CF568 conjugated Donkey Anti-Rat IgG (H+L) Antibody Biotium Cat#20092 Alexa fluorophore 488-conjugated Phalloidin Thermo Fisher Scientific Cat#A12379 Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) Jackson Immuno Research Laboratories Cat#111035144 Chemicals, Peptides and Recombinant Proteins Hoechst 33258 Thermo Fisher Scientific Cat#H3569 Tetramethylrhodamine-conjugated 2000 kDa Dextran Thermo Fisher Scientific Cat#D7139 Texas-Red conjugated 70 kDa Dextran Thermo Fisher Scientific Cat#D1830 Qtracker 655 vascular labels Thermo Fisher Scientific Cat#Q21021MP Carbomer 940 Spectrum Chemical Cat#C1477 Fasudil HCl (HA-1077) Alfa Aesar Cat#J60751 6-Carboxfluoreceine diacetate (CFDA) Sigma-Aldrich Cat#C5041 Experimental Models: Organisms/Strains Wildtype mice Harlan laboratories C57BL/6JHsd mice Lifeact-EGFP mice Laboratory of Roland Wedlich-Soldner, University of Münster, Germany ( Riedl et al., 2010 ) NA Software and Algorithms MotionTracking Morales-Navarrete et al., 2015 http://motiontracking.mpi-cbg.de Imaris BitPlane, Inc. NA OpenFoam NA http://www.openfoam.org Fiji Schindelin et al., 2012 https://fiji.sc ANSYS ICEM CFD Ansys, Inc. NA FitModel Zeigerer et al., 2012 http://pluk.mpi-cbg.de/projects/fitmodel Morpheus Starruss et al., 2014 https://gitlab.com/morpheus.lab/morpheus Matlab The MathWorks, Inc. NA GraphPad 7 GraphPad Software, Inc. NA Open in a separate window KEY RESOURCES TABLE The bile canaliculi network displays geometric heterogeneity within the liver lobule Bile flow is driven by osmotic effects and bile canaliculi contractility A multi-scale model reveals gradients of bile velocity and pressure within the lobule The multi-scale model predicts drug-induced alterations of bile flow
Techniques: Recombinant, Software
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